91九色视频

BiaCore
The Biacore S200 SPR instrument can measure interactions of various sample types, from low molecular weight drug candidates to high molecular weight proteins (also DNA, RNA, polysaccharides, lipids, cells, and viruses) in various sample environments (e.g., DMSO-containing buffers, plasma, and serum).

Director: Adriano Marchese, PhD; Manager: Ya Zhuo, PhD

The Biochemistry Mass Spectrometry shared equipment houses a Thermo LTQ Mass Spectrometer for walk-up use for peptide and protein analysis. It is available to Department of Biochemistry faculty, students and staff and their collaborators.

Director: John Corbett, PhD

The mission of FLOW is to provide investigators with: technical support and training for multicolor analytical flow cytometry and cell sorting; operator-assisted cell sorting in both standard and BSL-2+ modes; consultation for application, assay development and data analysis; and advanced data interpretation.

Director: Calvin Williams, MD, PhD; Manager: Galina Petrova, PhD

The Children’s Research Institute (CRI) Histology Core is a CAP accredited laboratory that offers a full range of routine and specialized histological, immunological and related services. Training is provided by ASCP certified Histologists with analysis from an American Board of Pathology certified Pathologist. We offer services to investigators from Children’s Hospital of Wisconsin, the 91九色视频 and other institutions.

Director CRI Cores: Jason Jarzembowski, MD, PhD; Scientific Director: Paula North, MD, PhD; Manager: Christine Duris BS, HTL, QIHC(ASCP)

Children’s Research Institute’s imaging core houses with a variety of state-of-the-art microscopic imaging systems including several confocal microscopes, a laser microdissection system and whole slide scanners. The imaging core is open to all investigators including those at our collaborating institutes. Core users will be trained and consulted on the instrumentation, software, and analysis.

Director CRI Cores: Jason jarzembowski, MD, PhD; Director: Suresh Kumar, PhD

The CRI Pediatric BioBank & Analytical Tissue Core provides quality-controlled banking of human pediatric tissue, blood, bone marrow, and other biological samples for use by investigators both internal and external to 91九色视频 and its affiliated institutions.

Medical Director: Jason Jarzembowski, MD, PhD; Scientific Director: Julie Tetzlaff, PhD

Advanced equipment for single cell (Chromium Controller, Chromium X) sequencing preparation and spatial genomics (Visium CytAssist) for self-use.

Director: Nick Kampa

The CVC Confocal Core offers training and use of a range of Nikon microscopes including laser scanning confocal, inverted and upright widefield equipment. The CVC iPSC provides culturing services of patient samples into iPSCs, differentiation, karyotyping, isolation and generation of specific cell types, and gene-editing, biobank of clinical-grade iPSCs.

Confocal Manager: Megan Harwig, PhD; iPSC Manager: Gracious Ross, PhD

The OCAM-EM encompasses both Light and Electron Microscopy services that is open to all 91九色视频 investigators, investigators at affiliated institutions and other non-91九色视频 investigators. The Electron Microscopy laboratory offers tissue processing, immunoelectron microscopy, negative staining, enzyme cytochemistry, ultrastructural electron tomography and TEM training. The Light Microscopy laboratory services include training for independent use and assisted use for spinning disc confocal microscopes and super resolution microscopy and 3D analysis software.

Director: Ashish Gadicherla, PhD; EM Manager: Rob Goodwin, Light Microscopy Manager: Megan Harwig, PhD

The microscopy core at the Neuroscience Research Center offers a variety of confocal imaging systems for specialized imaging of neuronal tissue, including the AxL Cleared Tissue LightSheet Microscope along with appropriate software including Bitplane Imaris 10.0 F1 package and SVI Huygens Professional deconvolution software. The availability of a cleared tissue instrument enables unparalleled visualization of developmental processes, injury recovery, and disease-related pathology.

Scientific Director: Suresh Kumar, PhD; Faculty Director: Chris Olsen, PhD

Macromolecular X-ray Crystallography Facility
The facility includes an X-ray diffraction system consisting of a Rigaku R-AXIS IV++ image plate detector system, MicroMax 007 generator, and automated crystallization system equipped with a CrysCam for nanoliter crystallization and visualization. The Macromolecular X-ray Crystallography Facility is open to 91九色视频 faculty members and offers a variety of training levels. 

Co-Directors: Dawn Wenzel and Francis Peterson

The CAP-accredited 91九色视频 Tissue Bank provides services involving human biospecimens to 91九色视频 faculty investigators. In addition to distributing tissues and other biospecimens, services associated with human biospecimens include tissue and blood processing, RNA/DNA extraction, tissue analytics, and storage. Biorepositories are maintained for General Tissue and Tumor Bank, the Maternal Bank, the COVID-19 Bank, and the 91九色视频 Cancer Center Immuno-oncology Bone Marrow Bank.

Medical Director: Steven Kroft, MD; Research Program Director: Mary Rau

91九色视频 Center for Microbiome Research (CMR) provides specialized resources for microbiome-focused studies and resources for microbial experiments including gnotobiotic and axenic rodent husbandry, and state-of-the-art microbial bioinformatics including 16S rRNA gene sequencing, microbial metagenomics and RNA sequencing, and spatial microbial transcriptomics.

Director: Nita Salzman, MD, PhD; Manager: Mary Holtz, PhD

The Mellowes Center provides short read (Illumina) and long read (Oxford Nanopore) sequencing (NGS), single cell sequencing and spatial genomic analysis, and NanoString nCounter applications to investigate the genome, transcriptome, epigenome and proteome. The Mellowes Center Bioinformatics Resource will assist in processing data and provides services or collaborative research support to 91九色视频 investigators. Analysis services include study design, data acquisition, data analysis, and publication. Standard and novel assays, and analyses, are applied to integrate new data types, in support of investigator single or multi-omics studies.

Research Director: Angela Mathison; Bioinformatics Director: Xiaowu Gai, PhD; Project Manager: Jaime Wendt Andrae

The Structural Biology Shared Resources (SBSR) facility is made up of two components. The protein production facility was developed to supply high quality homogeneous proteins for structural biology and other applications using a variety of expression systems including baculovirus/insect cell, HEK293, CHO, and E.coli. The CryoEM facility houses a Thermo Glacios 200kV Cryo-TEM configured to carry out single particle analysis in support of protein structure derivation.

Director: Wei Liu, PhD; Protein Production Manager: Yuanyuan Ma, PhD; Interim Technical Director: Ashish Gadicherla, PhD

The Translational Metabolomics Shared Resource (TraMSR) (Redox & Bioenergetics, Preclinical Biomedical Imaging, Mass Spectrometry, Radiology Quantitative Imaging) provides expertise and state-of-the-art instrumentation for 91九色视频 investigators and cancer center members to explore cancer and non-cancer metabolism by enabling users to run mass spectrometry analyses, bioenergetic and redox function analyses, pre-clinical imaging and therapy response.

Director: Karin Hoffmeister, MD

TraMSR Biomedical Imaging
The Biomedical Imaging Shared Resource (BISR) advances research at 91九色视频 by providing expertise and state-of-the-art technology and methods for preclinical bioluminescence and fluorescence, low dose X ray microCT and Cherenkov Luminescence imaging. Preclinical near-infrared (NIR) and short-wave infrared imaging (SWIR) greatly increasing the translational potential of non-cancer and cancer center research.

Director: Amit Joshi, PhD; Manager: Shayan Shafiee, PhD

TraMSR Mass Spectrometry
The TraMSR Mass Spectrometry Shared Resource provides multiple analytical services for proteins, peptides, lipids, small molecules, metabolites, complex glycans and other biological molecules to investigators at 91九色视频 and our partner institutions.

Director: Kazuhiro Aoki, PhD; Manager: Michaela Pereckas, BS

The Department of Radiology, in collaboration with the 91九色视频’s Clinical Cancer Center (91九色视频CC) Clinical Trials Office, supports the assessment of tumor response to therapy guided by board certified radiologists. The QIL support clinical trials using Precision Imaging Metrics software to track and maintain clinical trial response criteria.

Co-Directors: Peter LaViolette, PhD and Stephanie Vincent-Sheldon, MD; Quality Analysts: Maureen Levenhagen and Allie Lowman

TraMSR Redox and Bioenergetics
Metabolic services supported by the Redox and Bioenergetics Shared Resource (RBSR) include reactive oxygen species(ROS) measurement, metabolic pathway profiling, oxygen consumption, glycotic function, membrane potential and metabolic reprograming using Agilent Seahorse , HPLC and UHPLCelectron paramagnetic resonance (EPR) and luminescence spectrometers.

Director: Jacek Zielonka, PhD; Manager: Monika Zielonka, BS